PECCI Code (Python Estimation for Carbon Concentration and Isotopes) for Calculating the Concentration and Stable Carbon Isotopic Composition of Dissolved Inorganic Carbon (DIC) in Precipitation for northwestern Arkansas

In karst settings, hydrograph separations using isotopic tracers are commonly and effectively used to quantify the proportions of rain rapidly delivered to springs along fractures and conduits during storm events. Dissolved inorganic carbon (DIC) is an effective, non‐conservative tracer for use in hydrograph separations of karst waters because of the ubiquitous nature of carbon in the sources of waters to caves and springs and unique concentrations and isotopic compositions of carbon inputs. DIC concentration and isotopic composition (δ¹³C‐DIC) in rain are typically calculated based on atmospheric carbon dioxide (CO₂) using equilibrium carbonate reactions and stable carbon isotope fractionation values. As atmospheric CO₂ changes, traditional assumptions applied in attaining calculated values can result in error, and better estimates of rain DIC are needed. The concentration and isotopic composition of rain DIC in the karst of northwestern Arkansas was calculated using Python™ programming language based on local atmospheric CO₂ and rain pH data from 2011 to 2013. Python™ provides an open‐source code and rapid means to complete iterative calculations, and the PECCI code (Python™ Estimation for Carbon Concentration and Isotopes) can be used for rain DIC calculations in other areas. Measured northwestern Arkansas atmospheric CO₂ had a median concentration of 397.7 ± 4.3 ppm and increased slightly over three years and median δ¹³C‐CO₂ was ‐8.5 ±0.4 ‰. Rain samples exhibited a median pH of 5.6 ±0.4. Calculated rain DIC ranged from 0.17 to 0.34 mg/L and δ13C‐DIC ranged from ‐8.5‰ to ‐8.2‰ between 5 and 30 °C. At an average annual temperature of 14.6 °C, rain DIC was calculated to be 0.25 mg/L and δ¹³C‐DIC was ‐8.34 ‰. Although the variations in DIC are small, the concentration and isotopic composition of end‐member sources in hydrograph separations controls the final hydrologic budget calculations. The PECCI code can be modified to calculate rain DIC for otherstudy sites or time periods

Sensory Feedback, Error Correction, and Remapping in a Multiple Oscillator Model of Place-Cell Activity

Mammals navigate by integrating self-motion signals (“path integration”) and occasionally fixing on familiar environmental landmarks. The rat hippocampus is a model system of spatial representation in which place cells are thought to integrate both sensory and spatial information from entorhinal cortex. The localized firing fields of hippocampal place cells and entorhinal grid-cells demonstrate a phase relationship with the local theta (6–10 Hz) rhythm that may be a temporal signature of path integration. However, encoding self-motion in the phase of theta oscillations requires high temporal precision and is susceptible to idiothetic noise, neuronal variability, and a changing environment. We present a model based on oscillatory interference theory, previously studied in the context of grid cells, in which transient temporal synchronization among a pool of path-integrating theta oscillators produces hippocampal-like place fields. We hypothesize that a spatiotemporally extended sensory interaction with external cues modulates feedback to the theta oscillators. We implement a form of this cue-driven feedback and show that it can retrieve fixed points in the phase code of position. A single cue can smoothly reset oscillator phases to correct for both systematic errors and continuous noise in path integration. Further, simulations in which local and global cues are rotated against each other reveal a phase-code mechanism in which conflicting cue arrangements can reproduce experimentally observed distributions of “partial remapping” responses. This abstract model demonstrates that phase-code feedback can provide stability to the temporal coding of position during navigation and may contribute to the context-dependence of hippocampal spatial representations. While the anatomical substrates of these processes have not been fully characterized, our findings suggest several signatures that can be evaluated in future experiments

Representation of Non-Spatial and Spatial Information in the Lateral Entorhinal Cortex

Some theories of memory propose that the hippocampus integrates the individual items and events of experience within a contextual or spatial framework. The hippocampus receives cortical input from two major pathways: the medial entorhinal cortex (MEC) and the lateral entorhinal cortex (LEC). During exploration in an open field, the firing fields of MEC grid cells form a periodically repeating, triangular array. In contrast, LEC neurons show little spatial selectivity, and it has been proposed that the LEC may provide non-spatial input to the hippocampus. Here, we recorded MEC and LEC neurons while rats explored an open field that contained discrete objects. LEC cells fired selectively at locations relative to the objects, whereas MEC cells were weakly influenced by the objects. These results provide the first direct demonstration of a double dissociation between LEC and MEC inputs to the hippocampus under conditions of exploration typically used to study hippocampal place cells

The relationship between the field-shifting phenomenon and representational coherence of place cells in CA1 and CA3 in a cue-altered environment.

Subfields of the hippocampus display differential dynamics in processing a spatial environment, especially when changes are introduced to the environment. Specifically, when familiar cues in the environment are spatially rearranged, place cells in the CA3 subfield tend to rotate with a particular set of cues (e.g., proximal cues), maintaining a coherent spatial representation. Place cells in CA1, in contrast, display discordant behaviors (e.g., rotating with different sets of cues or remapping) in the same condition. In addition, on average, CA3 place cells shift their firing locations (measured by the center of mass, or COM) backward over time when the animal encounters the changed environment for the first time, but not after that first experience. However, CA1 displays an opposite pattern, in which place cells exhibit the backward COM-shift only from the second day of experience, but not on the first day. Here, we examined the relationship between the environment-representing behavior (i.e., rotation vs. remapping) and the COM-shift of place fields in CA1 and CA3. Both in CA1 and CA3, the backward (as well as forward) COM-shift phenomena occurred regardless of the rotating versus remapping of the place cell. The differential, daily time course of the onset/offset of backward COM-shift in the cue-altered environment in CA1 and CA3 (on day 1 in CA1 and from day 2 onward in CA3) stems from different population dynamics between the subfields. The results suggest that heterogeneous, complex plasticity mechanisms underlie the environment-representating behavior (i.e., rotate/remap) and the COM-shifting behavior of the place cell

Dominance of the proximal coordinate frame in determining the locations of hippocampal place cell activity during navigation.

The place-specific activity of hippocampal cells provides downstream structures with information regarding an animal\u27s position within an environment and, perhaps, the location of goals within that environment. In rodents, recent research has suggested that distal cues primarily set the orientation of the spatial representation, whereas the boundaries of the behavioral apparatus determine the locations of place activity. The current study was designed to address possible biases in some previous research that may have minimized the likelihood of observing place activity bound to distal cues. Hippocampal single-unit activity was recorded from six freely moving rats as they were trained to perform a tone-initiated place-preference task on an open-field platform. To investigate whether place activity was bound to the room- or platform-based coordinate frame (or both), the platform was translated within the room at an early and at a late phase of task acquisition (Shift 1 and Shift 2). At both time points, CA1 and CA3 place cells demonstrated room-associated and/or platform-associated activity, or remapped in response to the platform shift. Shift 1 revealed place activity that reflected an interaction between a dominant platform-based (proximal) coordinate frame and a weaker room-based (distal) frame because many CA1 and CA3 place fields shifted to a location intermediate to the two reference frames. Shift 2 resulted in place activity that became more strongly bound to either the platform- or room-based coordinate frame, suggesting the emergence of two independent spatial frames of reference (with many more cells participating in platform-based than in room-based representations)

Eber und Börge im Vergleich – Auswirkungen der Kastration auf agonistisches und sexuell motiviertes Verhalten unter ökologischen Haltungsbedingungen

Nach dem Verbot der betäubungslosen Ferkelkastration nach VO (EG) 889 (2008) zum 01.01.2012 stellt die Ebermast eine mögliche Alternative für die ökologische Schweinefleischerzeugung dar. Im Rahmen des Projektes „Ebermast im Verbund: Entwicklung eines Konzepts für die Produktion, Schlachtung, Verarbeitung und Vermarktung ökologisch erzeugter Eber entlang der Wertschöpfungskette“ ist es ein Ziel, das Ausmaß möglichen unerwünschten Verhaltens, wie agonistische Interaktionen und Aufreitverhalten der Eber, mit dem von Börgen unter praxisüblichen ökologischen Haltungsbedingungen zu vergleichen und wichtige Einflussfaktoren hierauf zu identifizieren. Auch mögliche Verletzungsfolgen sollen einbezogen werden. Aus einer Zwischenauswertung werden hier erste Ergebnisse zum Ausmaß des genannten Verhaltens und der Verletzungsfolgen vorgestellt. 9 Gruppen Eber und 7 Gruppen Börge wurden mit einem Lebendgewicht von etwa 80 kg auf vier ökologischen Praxisbetrieben beobachtet. Agonistische Interaktionen (Kopfschlagen, Beißen, Kampf und Besteigen ohne sexuelle Motivation) und Besteigen mit sexueller Motivation wurden mittels kontinuierlicher Verhaltenszählung (continuous behaviour sampling) jeweils über die ersten 15 Minuten einer jeden Lichtstunde von zusammenhängenden 48h-Aufnahmen ausgewertet. Zum selben Zeitpunkt wurden zusätzlich die Verletzungen bei den 109 Ebern und 91 Börgen gezählt. Eber zeigten bezüglich aller erhobener Verhaltensparameter gegenüber den Börgen signifikant höhere Häufigkeiten bzw. Dauern. Dagegen konnten hinsichtlich der Verletzungsscores zwischen Ebern und Börgen keine signifikanten Unterschiede festgestellt werden. Die Untersuchung wird fortgesetzt

Backward Shift of Head Direction Tuning Curves of the Anterior Thalamus: Comparison with CA1 Place Fields

SummaryThe head direction cell system is composed of multiple regions associated with the hippocampal formation. The dynamics of head direction tuning curves (HDTCs) were compared with those of hippocampal place fields. In both familiar and cue-altered environments, as a rat ran an increasing number of laps on a track, the center of mass (COM) of the HDTC tended to shift backward, similar to shifting observed in place cells. However, important differences existed between these cells in terms of the shift patterns relative to the cue-altered conditions, the proportion of backward versus forward shifts, and the time course of shift resetting. The demonstration of backward COM shifts in head direction cells and place cells suggests that similar plasticity mechanisms (such as temporally asymmetric LTP induction or spike timing-dependent plasticity) may be at work in both brain systems, and these processes may reflect a general mechanism for storing learned sequences of neural activity patterns

The frequency of item writing flaws in multiple-choice questions used in high stakes nursing assessments

Multiple-choice questions are a common assessment method in nursing examinations. Few nurse educators, however, have formal preparation in constructing multiple-choice questions. Consequently, questions used in baccalaureate nursing assessments often contain item-writing flaws, or violations to accepted item-writing guidelines. In one nursing department, 2770 MCQs were collected from tests and examinations administered over a five-year period from 2001 to 2005. Questions were evaluated for 19 frequently occurring item-writing flaws, for cognitive level, for question source, and for the distribution of correct answers. Results show that almost half (46.2%) of the questions contained violations of item-writing guidelines and over 90% were written at low cognitive levels. Only a small proportion of questions were teacher generated (14.1%), while 36.2% were taken from testbanks and almost half (49.4%) had no source identified. MCQs written at a lower cognitive level were significantly more likely to contain item-writing flaws. While there was no relationship between the source of the question and item-writing flaws, teachergenerated questions were more likely to be written at higher cognitive levels (p < 0.001). Correct answers were evenly distributed across all four options and no bias was noted in the placement of correct options. Further training in item-writing is recommended for all faculty members who are responsible for developing tests. Pre-test review and quality assessment is also recommended to reduce the occurrence of item-writing flaws and to improve the quality of test questions. © 2006 Elsevier Ltd. All rights reserved.postprin